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To inactivate LS GLP-1R neurons, we injected AAV expressing double-floxed EGFP and TeNT (AAV-DIO-EGFP-2A-TeNT) into the bilateral LS of GLP-1R-ires-Cre mice to achieve specific expression of EGFP-2A-TeNT in LS GLP-1R neurons (Figure 3A and Supplemental Figure 6C)
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You can also wait to drink water until after eating to take up less space in the stomach
